Validating Metagenomic Analyses Through Simulated Direct and Indirect Healthcare-Related Pathogen Transmission Events
Abstract: The prevalence of healthcare-acquired infections (HAI) places a significant economic burden on modern healthcare systems. Cultures are typically used to diagnose HAI; however, culture-dependent methods provide only limited presence/absence information and are not applicable to all pathogens. Next generation sequencing (NGS) has the potential to detect a wide variety... MORE
SigSci’s Austin TX DNA Forensics Laboratory Participated in an Interlab Study of STRmix™ Probabilistic Genotyping...
Title: STRmix™ collaborative exercise on DNA mixture interpretation Summary: An intra- and inter-laboratory study using the probabilistic genotyping (PG) software STRmix™ is reported. Two complex mixtures from the PROVEDIt set, analysed on an Applied Biosystems™ 3500 Series Genetic Analyzer, were selected. 174 participants responded. For Sample 1 (low template, in the... MORE
Title: A Systematic Method for the Targeted Discovery of Chemical Attribution Signatures – Application to Isopropyl Bicyclophosphate Production Abstract: Potential attribution signatures for the synthesis of a highly toxic bicyclophosphate, 4-isopropyl-2,6,7-trioxa-1-phosphabicyclo[2.2.2]octane 1-oxide (Isopropyl Bicyclophosphate or IPBCP) were discovered using a trilateral synthetic, analytical, and statistical approach. Initially, five synthetic routes were... MORE